Thursday, December 26, 2019

Identity And Image And Identity - 2008 Words

How one perceives the world around them is based on identity and images. Merriam Webster Online (2015) defines identity as â€Å"who someone is.† Image can be defined in two ways: â€Å"a mental picture, the thought of how something looks or might look† and â€Å"the idea that people have about someone or something† (Merriam Webster Online, 2015). Identity and image are so strongly intertwined that they both have influence over one another. Images can change how an individual perceives the identity of another person by allowing the individual to interpret and judge the image from their own selfish perspective. The identity of a person shapes how an image is created for them (Rezaei-Adaryani et al. 2012). These two concepts apply to any person and anything in this world. The nursing profession is just one possible option that has both a strong image and identity created for it. The nursing image is a concept with several different dimensions that includes four top ics: how the public views nursing, the nurse’s self-image (professional identity), how the nurse perceives the public’s views, and how the media characterizes or shows nursing (Rezaei-Adaryani et al. 2012). These four topics are very closely interrelated. How the media presents nursing affects the public’s image of nursing. This in turn affects how the nurse interprets how the public views them, which leads to the nurse’s personal self-image being changed or distorted. These four topics create a continuous cycle. If one topicShow MoreRelatedIdentity And The Self Image2176 Words   |  9 Pagesourselves? Other than the obvious components of identity, such as one s appearance, what else defines identity? How do others’ views affect the way we look at ourselves, and how do the groups we involve ourselves with define us? Lastly, how do these different components of identity intertwine, and how do they relate to the self-image we create of ourselves? T aking all of these questions into consideration, I am going to determine whether one s identity is transparent to others, or if it is am ambiguousRead MoreThe And Brand Identity / Brand Image Subjects994 Words   |  4 Pagesboth COO and Brand Identity/Brand Image subjects. Every successful research should be started with analysis of central themes as this will act as a basis for whole study. Taking into account this thinking, first part of this literature review will be dedicated to analyses of core aspects Brand Identity/Brand Image and Country-of-Origin Effect. Here you will be able to find discussion of history, background research and key factors of both topics. 2.1.1 Brand Image/Brand identity With the growth ofRead MoreMedia And Ethnic Identity Influencing Self Image1233 Words   |  5 Pagesthemselves. This research paper examines the ethnic media and the ethnic identity determining self-image than mainstream media does for young black women. Its seeks ways in which the black community declines stereotypes of color and beauty by increasing the groups of members and providing standards of what it means to be black and beautiful in this world. Much research has been made on the role of how media plays in people’s self-image. Majority of researches has been found on Caucasian women. As beingRead MoreSymbolism And Identity : `` Miraculous Images `` Renaissance Florence `` By Megan Holmes1423 Words   |  6 PagesMateriality and Identity Megan Holmes’s â€Å"Miraculous Images in Renaissance Florence† examines many of the ramifications of materiality. The materiality, an image’s physical properties, has direct impacts on the expression and popularity of immagini miracolose. These sacred images are subjects of miracles throughout the late 13th to 16th centuries. Two of the most important ramifications of materiality include the accessibility of the religious images and manifestation of the miracles. In this essayRead MoreFashion Defines Individual Identity, Image, Shoes, And Accessories965 Words   |  4 Pagesconfident and beautiful tonight because of that blue blouse. A new top or a new trend, society craves for fashionable outfits, shoes, and accessories. Fashion defines individual identity, impacts perceptions, and portrays a symbolic meaning. Fashion helps the individual grow and change one’s emotional and physical identities. The clothes one wears daily define how the individual feels and reflects one’s attitudes and moods. For example, if an individual is lethargic, one may be seen in sweatpants;Read MoreBody Image, Peer Pressure, and Identity in Mean Girls Essay1944 Words   |  8 Pagesmy adolescent years. I always remember the unintelligent things I did that I wish could change, but this Psychology class made me realize that all adolescents go through the same things I experienced. Adolescents are known to try to find their identity, go through peer pressure, make mistakes, and try new things. The move I picked that closely represented what adolescents go through was â€Å"Mean Girls†. Some of the scenes in the movie seem a little exaggerated, but it has happened in certain highRead More Medias Influence on Adolescent Girls Body Image, Identity and Sexuality2507 Words   |  11 PagesMedi as Influence on Adolescent Girls Body Image, Identity and Sexuality Missing Sources Cited nbsp;nbsp;nbsp;nbsp;nbsp;Gender Identity begins in early childhood when roles are given to children as to which toys they should play or not play with. A child’s body image is influenced by how people around her react to her body and how she looks. A pre-adolescent becomes more aware of what society’s standards are for the ideal body. The media has always had images of what the ideal woman should be. AsRead MoreIn What Ways Do Images Construct Identities? Discuss Using Specific Examples.2110 Words   |  9 PagesNAME: SEBASTIAN T ESSAY QUESTION 3 In what ways do images construct identities? Discuss using specific examples. In what ways do images construct identities? Discuss using specific examples. Stuart Hall defines identity as an ‘already accomplished fact, which the new cultural practices then represent’. We should think instead of ‘identity as a ‘production’ which is never complete, always in process, and always constituted within, not outside, representation’ (Hall 1994 p.392). An individual’sRead Moreunit 305 guide for social care worker that describes ways that the environment helps the well being and why it is important to support individuals that promote their identity, image and self esteem.786 Words   |  3 Pagesï » ¿ The link between identity, self Image and self esteem Identity - Characteristics and interests that somebody recognises as belonging uniquely to them and makes up their individual personality for life. Self Image - The opinion that you have of your own worth, attractiveness, or intelligence. Self Esteem - Confidence in your own qualities as an individual person. All of the above are linked. If a person feels that their identity is being repressed, by not being able to orRead MoreHow Authentic Images Of Artists Are Within The Music Industry1445 Words   |  6 Pages1. Discussion Intro: The following discussion will aim to answer the question of how authentic the images of artists are within the music industry. The question stemmed from research about identity work, in particular within the creative industries. Artists are faced with a lot of identity struggles and tensions which a lot of creative people encounter as well. The main prevailing issue or tension creative people constantly encounter that is unresolved is creativity versus commerce. This idea describes

Wednesday, December 18, 2019

atlanta compromise - 931 Words

Analytical Essay On The Atlanta Compromise Address Booker T. Washington is one of the most respected and influential African American figures in American history, Mr. Washington was born into slavery and was freed by the Emancipation Proclamation. His peaceful personality along with his exceptional education in many different studies turned him into a well-rounded individual. Washington wanted blacks and whites to become partners and strive for both races to succeed. He delivered his most famous speech in 1895 known as the â€Å"Atlanta Compromise†. In Atlanta Georgia, Mr. Washington expresses himself with exceptional rhetoric and tone throughout his speech. He states that African Americans should take benefit of what they know and endeavor†¦show more content†¦If the country seeks to be at the top right now that they will learn nothing and not remain there for long. He is saying a strong and successful nation or race learns how to build a good foundation understandi ng that it is up our next generations to understand what their responsibilities are to whatever they obtain. Booker T. Washington’s Atlanta Compromise Address is one of the most truthful and powerful speeches of our countries history. Although it was very short it was so powerful because of his great tone and rhetoric. His explained himself so well saying that African Americans shouldn’t try things they didn’t know how to do. They needed to learn the skills and that those skills would take time. They needed to stick to what they knew and build upon it so that their posterity may prosper. He also mentioned that whites need to change with adversity and accept what was happening at this time and try to work with the blacks instead of being stubborn and not. They needed to accept that blacks were defiantly like family when they were slaves. If both races overcame what they faced the country would become better and be aShow MoreRelatedAnalysis Of The Atlanta Compromi se Speech1112 Words   |  5 Pagestime. It affected many of the black society, including Booker T. Washington. He was a major contributor to the end of segregation, and in 1895, he delivered the Atlanta Compromise Speech. He believed that with the hard work of the blacks, that they would earn and gain the respect of the esteemed whites. The main idea of The Atlanta Compromise Speech was that blacks should obtain social responsibility and need to work from the bottom to top to achieve this. Booker T. Washington’s speech was given toRead MoreThe Atlanta Compromise Speech : Historical Context1716 Words   |  7 PagesThe Atlanta Compromise Speech Historical Context: Booker T. Washington was born a slave in 1856 and was nine years old when slavery ended. He became the principal of the Tuskegee Institute in Alabama, a school designed to teach blacks industrial skills. Washington was a skillful politician and speaker, and he won the support of whites in the North and South who donated money to the school. On September 18, 1895 Booker T. Washington gave an address to the Atlanta Cotton States and International ExpositionRead MoreThe Niagara Movement Speech And The Atlanta Compromise1796 Words   |  8 PagesBoth the Niagara Movement Speech and the Atlanta Compromise were written by some of the most well-educated and well-versed men in history, and while both men ultimately spoke to their respective audience about the absolute importance of racial equality, they took different paths in persuading their audiences to agree with their points. Mr. DuBois, in his Niagara Movement Speech, spoke about education, opportunity, and voting rights in an entirely logical way, and presented clear and concise ideasRead MoreThe Atlanta Compromise Address By Booker T. Washington Essay889 Words   |  4 PagesAtlanta Georgia 1895, Booker T. Washington would deliver a speech called the â€Å"Atlanta Compromise Address†. Influential speech made by Washington and at one point almost not allowed to be spoken, especially to an all white audience. However, having a black speaker would and should impress the Northerners and prove the racial changes in the south. Washington speech would provide the theory of â€Å"cast their buckets where they are† for all blacks. Beginnings to the end of Washington address, WashingtonRead MoreComparasion of Frederick Douglasss 4th of July Speech Booker T. Washingtons Atlanta Compromise Speech1210 Words   |  5 Pagesinhumane cruelty American attempted to conceal through its mockery. In contrast to Douglass, Washington’s famous speech known as the â€Å"1865 Atlantic Compromise† was not a protest or challenge of the political system, nor did he speak about the lack of social equality. Instead he focused his efforts on what blacks could accomplish, how they could compromise. He believed the militant rhetoric of Douglass and others distracted blacks from the path of liberty, equality, and economic success. Washington’sRead MoreBooker T Dubois Compare And Contrast Essay1110 Words   |  5 PagesCompromise vs. Conflict The stark differences between Booker T Washington and W.E.B. DuBois and their approaches to racism Do you believe that slow and steady wins the race, or that when talking about civil rights the sooner the better? Booker T. Washington and W.E.B. DuBois were two very influential people who fought for the rights of African Americans in the late 1880’s to early 1900’s. Although both men were fighting for the same thing, their views on how to approach equality and their personalRead MoreBooker T. Washington, W.E.B. Du Bois and John Hope Essay1286 Words   |  6 Pagesnamed Booker T. Washington gave a speech that would be later named the Atlanta Compromise at the Cotton States and International Exposition in Atlanta on September 18, 1895 (Booker T. Washington Biography). Booker T. Washington was born in to slavery and would eventually gain his freedom after the Civil War was over. He was biracial, but never knew who his white father was (Booker T. Washington Biography). His â€Å"Atlanta Compromise† speech was very controversial amongst the African American commun ityRead MoreGranting The Rights Of African Americans : Reconstruction Period1519 Words   |  7 Pagesmaterial wealth. As Straker states in his essay â€Å"The South s progress, socially, is only apparent and shadowy; it is not substantial; it cannot be with a divided and unequal people in condition and opportunity†.[3] Booker T Washington, in The Atlanta Compromise (1985) mentions that in the face of discriminatory laws against the Blacks, chances of their development materially or politically was very less. The speech that he gave in front of a predominantly white audience highlighted much on the aspectsRead More Booker T. Washington and W.E.B. Du Bois Influences on Equality594 Words   |  3 Pagesinto the civil rights movement, but in very different customs. Booker T. Washington and W.E.B. Du Bois both were huge influences to civil rights, one founding what we know today as the NAACP and the other spoke of a philosophy known as the Atlanta Compromise. Booker T. Washington has a compelling story of sorts. He was born into slavery and climbed his way out of poverty. He received his education at the Hampton Institute and encouraged other blacks to do the same as him. Soon after he becameRead MoreComparing and Contrasting the Ideolodies of Booker T. Washington and W.E.B Du Bois1053 Words   |  5 Pagesin social change. Unlike Du Bois, Washington believed that African Americans should accept discrimination for the time being and elevate themselves through hard work and economic gain to win the respect of whites. . Washington stated in the Atlanta Compromise speech, interlacing our industrial, commercial, civil, and religious life with yours in a way that shall make the interests of both races one...we can be as separate as the fingers, yet one as the hand in all things essential to mutual progress

Monday, December 9, 2019

Othello Treatment of Women Essay Example For Students

Othello Treatment of Women Essay Throughout history, the treatment of women has been an ever-changing issue. Othello by William Shakespeare is a story in which the women characters are treated in the unfair way that women of the time of the story were treated. This makes the story a great model for comparison of the treatment of women in the present time and in the past time when the story takes place. In order to make this comparison, one must first examine the way that the characters of Bianca, Emelia, and Desdemona are treated. Everyone including her husband, Cassio, treats the character of Bianca unfairly. This is because Bianca is really in love with Cassio, however he can only see the relationship as being a physical one. Due to this, Cassio has no problem making fun of Bianca behind her back when she leaves, and then wooing her when she returns again. The character of Emelia is an even harder one to evaluate. This is because she is the smartest woman character, but nonetheless men still seem to find a way to treat her poorly. When Emilia goes out of her way to help her husband Iago do evil, he barely even thanks her. Desdemona is the last of the characters to evaluate, however she is also the most important. Desdemona is treated very unfairly because she is a faithful wife, who loves her husband very much. However, through the help of Iago, her husband, and Othello suspects Desdemona is cheating on him. This is not true, but still Othello believes his friend long before he would listen to the woman who loves him. Othello then, instead of treating Desdemona fairly in return for her love, argues with and makes fun of her. Othello is a great model to be used to compare the treatment of women then and now because by reading it, one could see how much worse the treatment of women was then than it is now.

Monday, December 2, 2019

Performing an EKG

Performing an EKG Training Proposal Proposal Context This instructional training will focus on performing an EKG/ECG. Oftenly, many EKG technicians don’t make an ECG properly. More often than not mistakes are made and leads of the equipment are switched. When this happens, technicians and medical professionals come to wrong conclusions.Advertising We will write a custom coursework sample on Performing an EKG specifically for you for only $16.05 $11/page Learn More According to Leshin et al (1992, p.224) one of the prevalent mistakes in the performance of an ECG is the switching of the right and left arms. The result normally is negative complexes that occur in I that lead to an indication of deviation at the right axis. Lead switching by EKG learners is common and the trend serves to show a gap in the skills that are needed by these professionals for optimal performance. When wrong conclusions are made, from ECG readings, the most likely outcome i s a wrong diagnosis. The effects of wrong diagnosis are far reaching and may include loss of life for patients and career-enders for professionals (Michelle, E.H. et al. (2009, p.331). Besides, it does nothing to contribute to the development of EKG performance and the profession generally. It’s for the above reasons that this instructional training will come in handy to ensure that the gap in skills is bridged and technicians and medical professionals perform optimally in setting up EKG equipment. The course will be mandatory for all learners in the remedial program and optional for other learners who might feel the need to polish their skills in EKG reading and analysis. Knowledge/Skill Gap Actual performance EKG learners make many lead switching mistakes while learning. These mistakes include the reversal of right leg and right arm. Normally this leads to the difficulty in seeing signals in lead II. There is also the reversal of the right and left arm electrodes by learner s. This mistake commonly happens through the reversal of leads II and III as well as leads aVR and aVL. Another mistake according to Kathleen,G. et al. (2011, p. 331) that learners make involves the reversal of the left arm and left leg. When this is done, it takes place through the reversal of leads I and II, aVR and aVF and the inversion of lead III. Reversal of the right arm and left leg is another common mistake that EKG learners make. The mistakes involves the inversion of leads I, II and III and leads aVR and aVR. This skills gap was identified through observation of test data of the learners and learner performance in the classroom. There was a consistent pattern in test results where learners made the mistakes recurrently, a clear indication that there is a knowledge gap that needs to be addressed by training.Advertising Looking for coursework on health medicine? Let's see if we can help you! Get your first paper with 15% OFF Learn More Desired performance Lea rners after this training will be expected to correctly read the signal, connect, and record through the ISO-1 isolated input amplifier unit. The learners will also be able to correctly use the four plate electrodes and the associated straps, the electrolyte paste of the tube and alcohol pads. It is expected that learners will be able to correctly perform the procedure and achieve correct results in EKG reading. They will be expected to correctly place the subject in the correct position and perform a successful ECG reading and analysis. This learning module is necessary because it will help eliminate the common mistakes that learners make in EKG classes. Data suggests that mistakes of this nature occur every year among EKG learners. This model therefore will come in hand in enhancing training in this particular area for both current and future learners. Failure to develop this module will lead to release of half-baked EKG technicians and Proffesionals to the market that will lead t o a compromise of standards in the healthcare industry. Besides, it will not be in the best interest of the training organization if its graduates fail to meet industry standards. Instructional goal The main drive behind the development of this module is the achievement of consistency in the performance of EKG learners. It’s assumed after the development and implementation, the common mistakes described above will be significantly reduced among learners after the initial training is done. The module is also expected to eventually identify the leading causes of the mistakes made by learners so that programs can be put in place to address the. Additionally, the program is expected to help learners not in remedial classes to polish and refresh their skill. A proposal will be fronted to the institution for the training module to be made permanent to ensure continued adherence of organizational standards. Objectives Terminal objectives Learners will be tested at the end of the cou rse and they must meet the objectives that will be set before the beginning of the course. Testing will compulsory for remedial learners and optional for others. The objectives include:Advertising We will write a custom coursework sample on Performing an EKG specifically for you for only $16.05 $11/page Learn More To test remedial learners on the placement of the right leg and right arm to achieve signal reading in lead II. Learners will be graded and one will be deemed to have passed upon achieving the above signal and a score of over 80 accuracy will indicate achievement of consistency To test the placement of right and left arm electrodes. Learners will be deemed to have achieved consistency after correct placement of leads II, III, aVR, and aVL. An accuracy score of 80% by remedial learners will indicate achievement of consistency. To test the placement of left arm and right leg. Correct placement of leads I and II, aVR and aVF and lead III and a s core of over 80% by remedial learners will indicate consistency in the learner. To test the placement of the right arm and left leg. Correct placement of leads I,II and III and aVR and aVF and a score of over 80% by the remedial learners will signify consistency Enabling Objectives Instructors will be aiming to achieve the above through enabling objectives that will be: To teach learners on the correct placement of the right leg and right arm to ensure a signal is occurrence in lead II To teach learners on how to avoid reversal of leads I and III as well as leads aVR and aVL that result in incorrect placement of right and left arm electrodes. To teach learners on how to avoid reversal of leads II, and I leads aVR and aVF and the inversion of lead III to avoid incorrect placement of the left arm and the left leg. To teach learners on how to avoid inversion of leads I, II, and III and also leads aVR and aVF so as to ensure correct placement of the right arm and left leg. Content an d Tasks The mistakes that have highlighted the knowledge gap in as said before deal with lead switching. The course content therefore will emphasize on the information that the learners will need to make correct connections to avoid switching. Its important for the learners to be well versed in Cardiac Conduction System Anatomy, cardiac physiology and in the theory of electrocardiography. The content will specifically focus on lead connection and EKG interpretation. A wide variety of topics will be offered in one moth course that will include information on the administration of EKG and alternate EKG types, indication of EKG and EKG overview. a combination of various instructional methods will be used including text, photography, and other interactive programs that will be used to suit the different learners; preferences. Presentation will mainly be carried out using the Microsoft PowerPoint and a digital scanner that will necessitate the direct input of EKG. Learners will be assign ed both in groups real life cases where they will be required to input their skills on EKG performance. At the end of every session the lesson content will be distributed to the learners through a multimedia on a CD ROM to enable them go through the content to develop perfection. Target Audience Description This learning module will be targeting all learners in remedial learning program in the institution. Besides, the program will be open to other learners who will be in need of polishing their EKG skills. A number of factors that will either help or inhibit the rate of understanding or learning of the learners will affect delivery of this program.Advertising Looking for coursework on health medicine? Let's see if we can help you! Get your first paper with 15% OFF Learn More Their mental abilities and level of knowledge in the EKG theory will be big factors in learning. Besides there will need to be motivation for the students to help them grasp the concepts that will be necessary for consistent performance of EKG procedures. Additionally, learners will be expected to be well versed in both basic and advanced computer skills that will come in handy in manipulating some EKG equipment and software. Project Scope Success of this model will largely depend on the availability of funds. Given the sophisticated type of equipment that is needed for its success and the need for a clear presentation, the module will rely greatly on designers and other information technology components that will only be availed through monetary resources. Considering the institution’s situation financially, the module is likely to be met with financial difficulties. Presentation will be carried out in PowerPoint presentations using a projector. It will incorporate colored i mages and vide clips that will help learners to relate with the content of that will be presented. The classes will also include lab sessions where learners will demonstrate their skills. The sessions will offer a chance for instructors to gauge the level at which learners will demonstrate their theory knowledge to practical. It will assist instructors to ascertain the knowledge gaps and that exists in the approaches the learners use and the actual performance of EKG’s. Technological Considerations There will be a lot of technological involvement in the program. Those learners that won’t be well versed in IT will be taken through a short refresher course while course-designing instructors will assist learners in navigating the EKG equipment that will be used for practical purposes. References Kathleen,G. et al. (2011). Integrating technology in nursing education: Tools for the knowledge era.Ontario: Jones Barlette Learning. Leshin, B.C et al. (1992). Instructional des ign strategies and tactics. New Jersey : Educational technology Publications. Michelle, E.H. et al. (2009). Clinical Medical Assisting: A Professional, Field Smart Approach to the workplace. New York: Cengage Learning. This coursework on Performing an EKG was written and submitted by user Annabella K. to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Wednesday, November 27, 2019

Bacterial Transformation with pGLO Essay Essays

Bacterial Transformation with pGLO Essay Essays Bacterial Transformation with pGLO Essay Essay Bacterial Transformation with pGLO Essay Essay Aim:Practice formulating hypotheses. anticipations. and experimental design. ? Describe the rules of bacterial transmutation.Explain the process for cistron transportation utilizing plasmid vectors. ? Induce the transportation of the pGLO cistron ( in a plasmid ) into E. coli. ? Describe the traits carried by the pGLO cistron.Describe how to trip ( â€Å"turn on† ) the pGLO cistron.Describe how to acknowledge the transformed cells ( from this lab ) . ? Know the footings used in this lab including transmutation ( in this instance transmutation does NOT intend the transition of a normal cell to a cancerous one ) . vector. plasmid. fluorescence. antibiotic opposition. E. coli.Answer the inquiries posed in this lab. PRELAB:Read about the control of cistron look on pages 353-356 and about transmutation on page 348 of the text edition.Read this lab and be ready to get down the exercisings.Specify the undermentioned footings ( but do non manus in ) : transmutation. vector. plasmid. fluorescence. antibiotic opposition. E. coliIntroduction:In this lab you will execute a process known as a familial transmutation. Remember that a cistron is a piece of DNA that provides the instructions for doing ( coding for ) a protein that gives an being a peculiar trait. Familial transmutation literally means alteration caused by cistrons and it involves the interpolation of a cistron ( s ) into an being in order to alter the organism’s trait ( s ) . Familial transmutation is used in many countries of biotechnology. In agribusiness. cistrons coding for traits such as hoar. plague. or spoilage opposition can be genetically transformed into workss. In bio-remediation. bacteriums can be genetically transformed w ith cistrons enabling them to digest oil spills. In medical specialty. diseases caused by faulty cistrons are get downing to be treated by cistron therapy ; that is. by genetically transforming a ill person’s cells with healthy transcripts of the cistron involved in their disease. You will utilize a process to transform bacteriums with a cistron that codes for a Green Fluorescent Protein ( GFP ) . The real-life beginning of this cistron is the bioluminescent jellyfish Aequorea Victoria. The cistron codifications for a Green Fluorescent Protein that causes the Portuguese man-of-war to fluoresce and glow in the dark. Following the transmutation process. the bacteriums express their freshly acquired jellyfish cistron and bring forth the fluorescent protein that causes them to glow a brilliant green colour under UV visible radiation. In this activity. you will larn about the procedure of traveling cistrons from one being to another with the assistance of a plasmid. In add-on to one big chromosome. bacteriums of course contain one or more little round pieces of DNA called plasmids. Plasmid DNA normally contains cistrons for one or more traits that may be good to bacterial endurance. In nature. bacteriums can reassign plasmids back and Forth. which creates the chance for them to portion these good cistrons. ( Note that the bacteriums don’t know that they are picking up good genes. ) This natural mechanism allows bacteriums to accommodate to new environments. The recent happening of bacterial opposition to antibiotics is due to the transmittal of plasmids. The alone plasmid we use encodes the cistron for the Green Fluorescent Protein ( GFP ) and a cistron for opposition to the antibiotic. Principen. The plasmid besides incorporates a particular cistron ordinance system. which can be used to command look of the fluorescent protein in transformed cells. The cistron for the Green Fluorescent Protein can be switched on in transformed cells by adding the sugar. arabinose ( Ara ) . to the cells’ alimentary medium. Choice for cells that have been transformed with the plasmid DNA is accomplished by growing on antibiotic home bases. Transformed cells will look white ( wild type phenotype ) on home bases non incorporating arabinose. and fluorescent green under UV light when arabinose is included in the alimentary agar. You will be provided with the tools and a protocol for executing familial transmutation in Escherichia coli. This transmutation process involves three chief stairss. These stairss are intended to present the plasmid DNA into the E. coli cells and supply an environment for the cells to show their freshly acquired cistrons. Many species of bacteriums have particular membrane proteins for the consumption of Deoxyribonucleic acid from the external environment. E. coli does non look to hold these types of membrane proteins ; nevertheless. puting E. coli in a comparatively high concentration of Ca ions and executing a process called â€Å"heat shock† will excite these cells to take up pieces of foreign DNA. To travel the plasmid DNA through the cell membrane you will:1. Use a transmutation solution of CaCl2 ( calcium chloride )2. Transport out a process referred to as heat dazeFor transformed cells to turn in the presence of Principen you must:1. Supply them with foods and a short incubation period to get down showing their freshly acquired cistronsRead the lab exercising and follow the waies carefully. You will make this lab in lab groups of 3-4 pupils. Completion of this portion of the lab will take 2 lab periods ( or 1 lab and 1 category ) . In the 2nd lab period you will analyse your consequences. Part I: BACTERIAL TRANSFORMATION Exercise A: Introduction to Sterile Technique ( in lab session ) You will pattern utilizing unfertile technique. as instructed at the beginning of lab session. before you do the experiment. When culturing bacteriums. you must non present other. polluting bacteriums into your civilization. Potentially polluting bacteriums are omnipresent ; they are found everyplace ( including on the bench top and on your custodies ) . It is particularly of import to maintain the vaccination loops. the pipette tips. and the surfaces of the agar home bases must non touch or be touched by any polluting surface. Exercise Bacillus: Bacterial Transformation ( in lab session )MATERIALS A ; PROCEDURES1. Follow the processs in the â€Å"Transformation Kit-Quick Guide† provided in lab. 2. The home bases will be incubated for 24-48 hours. and so placed in a icebox to decelerate the growing of the bacterium. You will than detect the home bases in the following lab period to roll up your informations. 3. Complete your lab study ( see following page ) : explicate a hypothesis on which this probe is based. of how E. colicells can be transformed by the pGLO plasmid. formulate the anticipations. and explain the experimental design.LAB 9: TO TURN INAnswer the inquiries and fill in the tabular array in the infinite provided below. Complete the Hypothesis. Predictions. and Experimental Design subdivisions during the first lab period. The Results subdivision will be completed after we analyze the informations next hebdomad. Hypothesis Formulate a hypothesis on which this probe is based. of how E. coli cells can be transformed by the pGLO plasmid.PredictionsPrepare and finish the tabular array below to bespeak what you predict will go on on each of the four agar home bases. ( Will E. coli grow on these home bases? Will the E. coli have any particular belongingss compared to wild type? ) Plate Plasmid? Growth ( G )No Growth ( NG )Other belongingss?LB/amp +DNALB/amp/ara +DNALB/amp -DNALB -DNANote: LB is the alimentary mixture that is added to the home base agar to feed the bacterium. Experimental designExplain the experimental design:1. What is/are the independent variable ( s ) in this experiment?2. What is/are the dependant variable ( s ) ?3. Which plates will function as control home bases? Do you anticipate cells to turn on these home bases? Why or why non? What is the intent of these controls?4. Define plasmid.Part II: ANALYZING THE RESULTSConsequences1. In the tabular array below. fill in your observations after analyzing your home basesunder both normal and UV visible radiation.Plate Plasmid? Number ofColonies?Other belongingss?LB/amp +DNALB/amp/ara +DNALB/amp -DNALB -DNA2. Be your familial transmutation successful? How do you cognize?3. Be your consequences consistent with the anticipations you made in the tabular array on the old page? If non. why?4. See the following two braces of home bases. What do the consequences obtained from these home bases tell you about your experiment?a. -DNA LB and -DNA LB/ampB. +DNA LB/amp and -DNA LB/amp5. After analyzing your consequences. would you revise your hypothesis? If so. repeat your hypothesis below.

Saturday, November 23, 2019

The Historical Importance of Orville Wright

The Historical Importance of Orville Wright Why Is Orville Wright Important?: Orville Wright was one-half of the aviation pioneers known as the Wright Brothers. Together with his brother Wilbur Wright, Orville Wright made history with the first-ever heavier than air, manned, powered flight in 1903. Orville Wright: Childhood Orville Wright was born on August 19, 1871, in Dayton, Ohio. He was the fourth child of Bishop Milton Wright and Susan Wright. Bishop Wright was in the habit of bringing small toys home to his children after traveling on church business and it was one of these toys that Orville Wright attributed for his early interest in flight. It was the miniature Penaud helicopter that Milton Wright brought home in 1878, a popular mechanical toy. In 1881, the Wright family moved to Richmond, Indiana, where Orville Wright took up kite building. In 1887, Orville Wright started at Dayton Central High School, however, he never graduated. Interest in Printing Orville Wright loved the newspaper business. He published his first newspaper together with his friend Ed Sines, for their eighth-grade class. By sixteen, Orville worked summers in a print shop, where he designed and built his own press. On March 1, 1889, Orville Wright began publishing the short-lived West Side News, a weekly newspaper for West Dayton. Wilbur Wright was the editor and Orville was the printer and publisher. The Bicycle Shop In 1892, the bicycle had become very popular in America. The Wright Brothers were both excellent bicyclists and bicycle mechanics and they decided to start a bicycle business. They sold, repaired, designed, and manufacture of their own line of hand-built, made-to-order bicycles, first the Van Cleve and the Wright Special, and later the less expensive St Clair. The Wright Brothers kept their bicycle shop until 1907, and it was successful enough to fund their flight research. The Study of Flight In 1896, German flight pioneer, Otto Lilienthal died while testing his latest single-surface glider. After reading extensively and studying bird flight and Lilienthals work, the Wright brothers were convinced that human flight was possible and decided to conduct some experiments of their own. Orville Wright and his brother began experimenting with wing designs for an airplane, a biplane that could be guided by warping the wings. This experiment encourages the Wright brothers to proceed with constructing a flying machine with a pilot. Airbourne: December 17, 1903 On this day Wilbur and Orville Wright made the first free, controlled, and sustained flights in a power-driven, heavier-than-air machine. The first flight was piloted by Orville Wright at 10:35 A.M., the plane stayed twelve seconds in the air and flew 120 feet. Wilbur Wright piloted the longest flight that day in the fourth test, fifty-nine seconds in the air and 852 feet. After Wilbur Wrights Death in 1912 Following Wilburs death in 1912, Orville carried their legacy alone towards an exciting future. However, the hot new arena of aviation business proved volatile, and Orville sold the Wright company in 1916. He built himself an aeronautics laboratory and returned to what had made he and his brother so famous: inventing. He also stayed active in the public eye, promoting aeronautics, inventing, and the historic first flight that he made. On April 8, 1930, Orville Wright received the first Daniel Guggenheim Medal, awarded for his great achievements in aeronautics. The Birth of NASA Orville Wright was one of the founding members of NACA aka National Advisory Committee for Aeronautics. Orville Wright served on NACA for 28 years. NASA aka National Aeronautics and Space Agency was created from the National Advisory Committee for Aeronautics in 1958. Orville Wrights Death On January 30, 1948, Orville Wright died in Dayton, Ohio, at the age of 76. The home Orville Wright lived in from 1914 until his death, he and Wilbur planned the design of the house together, but Wilbur passed away before its completion.

Thursday, November 21, 2019

Major Depressive Disorder Case Study Example | Topics and Well Written Essays - 1500 words

Major Depressive Disorder - Case Study Example She feels worse in the mornings, guilty and hopeless, with suicidal ideation and has developed increased psychomotor retardation. She thinks she is ruining her family, who would be better off without her. These signs and symptoms indicate that she has Major Depressive Disorder (MDD), as defined by criteria in the Diagnostic and Statistical Manual of Mental Disorders, 4th edition, text revision (DSM-IV-TR) (American Psychiatric Association [APA], 2000). The criteria state that more than one important aspect of MDD, such as sustained depressed mood, anergia, anhedonia, disturbed appetite and sleep, psychomotor retardation, suicidal thought, negative thought, hopelessness, or poor concentration, should be present for no less than two weeks and not related to somatic effects, caused by substance abuse or adverse reactions to medication. Depression is the most widespread disorder in relation to mental health. According to World Health Organisation [WHO] (2001), people suffering from depression account for 5 to 10% of the population. The 'Bettering the Evaluation and Care of Health' survey (AIHW, 2007) reported that it accounted for 33.7% of mental health disorders managed by general practitioners in Australia in 2003-04. Most studies show that the development of MDD has a higher incidence in females than males in their life time, being found in females twice as much as in males (APA, 2000; Varcarolis, 2006, pp.326-327; WHO, 2001). Typically, MDD is more likely to occur in females aged between 20 and 40 years old (WHO, 2001). Causes assumed include responsibilities of multi-tasking, for instance, domestic and office work, wife and parenting roles, or the menses might contribute to the prominent prevalence of depression in females (APA, 2000; WHO, 2001). However, race, earnings, education or matrimony are not related to the frequency of MDD (APA, 2000; Varcarolis, 2002. p.454; WHO, 2001). 2. Pathophysiology of depression: Depression arises due to multifactorial causes, including biochemical, cognitive, hereditary and environmental factors, and sleep abnormalities (Varcarolis, 2002, pp.456-460; Varcarolis, 2006, pp.330-333). In Isabel's case, a reasonable assumption would be that the major pathophysiologic causes might be biochemical, cognitive factors, and sleep abnormality. There are many studies to indicate that biochemical imbalance can cause depressive disorders, in relation to certain neurotransmitters, mainly serotonin, noradrenaline and dopamine in the brain, which are known to play a role in the regulation of mood (Varcarolis, 2002, pp.456-460; Varcarolis, 2006, pp.330-333). The dysfunctional regulation of serotonin is related to decreased sex drive, appetite and sleep disturbance, as serotonin is particularly important in controlling sleep, appetite and sexual drive (Varcarolis, 2006, p.331; Zarate & Charney, 2003, pp.464-468). Reduction of noradrenaline can lead to loss of energy, interest, concentration, and decreased sexual drive (Varcarolis et al., 2006, pp.460-464; Zarate & Charney, 2003, p.464-468). The depletion of dopamine can be associated with loss of motivation and interest, because it contributes to the stimulation of the system of circuits of motivational behaviour in